Mouse monoclonal antibody against the human integrin aIIbß3 complex (platelet GPIIb/IIIa; CD41 / CD61 — the platelet fibrinogen receptor), clone CRC64, 0.2 mg. Complex-specific — recognises the intact aIIbß3 heterodimer but NOT the dissociated subunits. For platelet-function studies, flow cytometry, ELISA, Western blot and immunohistochemistry. Complements the platelet-surface markers cat. 136 (CD31) and cat. 192 (CD36).
SPECIFICATIONS
Catalogue number: 751 | Host: Mouse | Target: Human
Target antigen: Integrin aIIbß3 complex (platelet GPIIb/IIIa; CD41 / CD61), intact heterodimer
Gene: ITGA2B (CD41) / ITGB3 (CD61) | UniProt: P08514, P05106
Clonality: Monoclonal | Clone: CRC64 | Conjugate: Unconjugated
Specificity: Specific for the intact aIIbß3 complex; does not recognise the dissociated a or ß subunits.
Purification: Purified from ascites by DEAE chromatography.
Format: Lyophilised (mannitol, dextran, salts) | Package size: 0.2 mg (reconstitute to 1 mL)
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- Flow cytometry — platelet aIIbß3 (CD41 / CD61) surface detection.
- ELISA — aIIbß3 complex detection.
- Western blot — GPIIb/IIIa detection; the epitope is complex-dependent, so non-dissociating conditions are preferred.
- Immunohistochemistry — platelet and megakaryocyte identification in tissue.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Platelet biology and thrombosis — the aIIbß3 fibrinogen receptor.
- Glanzmann thrombasthenia research — aIIbß3 deficiency and dysfunction.
- Antiplatelet (GPIIb/IIIa inhibitor) pharmacology.
- Megakaryocyte and platelet-lineage identification.
