Mouse IgG1 monoclonal antibody against human alpha-fetoprotein (AFP), clone 5A6, 0.5 mg. Specific for human AFP with no reactivity to human albumin — its closest structural homologue. For AFP ELISA, dot blot, Western blot and immunohistochemistry: hepatocellular-carcinoma and germ-cell-tumour marker research, and fetal / maternal AFP studies.
SPECIFICATIONS
Catalogue number: 769 | Host: Mouse | Target: Human
Target antigen: Alpha-fetoprotein (AFP), the ~70 kDa fetal serum glycoprotein
Gene: AFP (4q13.3) | UniProt: P02771
Clonality: Monoclonal | Clone: 5A6 | Isotype: Mouse IgG1 | Conjugate: Unconjugated
Specificity: Specific for human AFP; no reactivity to human albumin.
Purification: Sodium sulphate precipitation and anion-exchange chromatography (DEAE-cellulose).
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M NaCl, 1% dextran, 1% mannitol | Package size: 0.5 mg
STORAGE / RECONSTITUTION
Reconstitute with sterile pure water per the supplied protocol. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — AFP capture or detection.
- Dot blot — AFP detection.
- Western blot — detection of the AFP band.
- Immunohistochemistry — AFP in liver, hepatocellular carcinoma and germ-cell-tumour tissue.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Hepatocellular-carcinoma research — AFP as the classical HCC tumour marker.
- Germ-cell tumour research — yolk-sac / endodermal-sinus AFP expression.
- Fetal and maternal serum AFP studies.
- AFP immunoassay development and hepatic-differentiation (iPSC / hepatocyte) markers.
