Porcine collagen types I and III (atelocollagen), extracted from washed dissected tissue by pepsin digestion in dilute acetic acid and purified by differential salt precipitation, supplied sterile, lyophilised and salt-free, 25 mg. Telopeptide-depleted (atelo) collagen that dissolves in dilute acetic acid and reassembles into a fibrillar 3-D gel on neutralisation at 37 °C. Use as a cell-culture coating substrate and 3-D gel matrix — the mixed type I/III blend complements the pure native porcine type I preparation cat. 882 and the 30 mg porcine atelocollagen cat. 130, and pairs with the anti-porcine collagen type I + III antibody cat. 386 for detection.
SPECIFICATIONS
Catalogue number: 785 | Product: Collagen type I and III (porcine, atelocollagen)
Source: porcine tissue
Purity: porcine collagen type I >90%; porcine collagen type III <10%. Passed sterility testing
Format: lyophilised, salt-free; sterile
Purification: extraction from washed dissected tissue with pepsin in dilute acetic acid, followed by differential salt precipitation. Pepsin treatment removes the non-helical telopeptide regions (atelocollagen), reducing immunogenicity and improving solubility
Package size: 25 mg
STORAGE / RECONSTITUTION
Reconstitution: dissolve in 10–50 mM acetic acid to a collagen concentration of 1–5 mg/mL overnight, using gentle mixing at +4 °C.
Shipped at ambient temperature.
Lyophilised protein: store at −20 °C or lower.
Reconstituted protein: store at +4 °C for up to 1 month.
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- Coating material for cell-culture studies — tested on human HepG2 cells, primary rabbit corneal keratinocytes, rabbit corneal endothelial and epithelial cells, mouse embryonal fibroblasts and mouse peritoneal macrophages.
- 3-D gel formation for three-dimensional culture, organoid and spheroid work.
- Protein standard for collagen type I / III ELISA and Western blot — pair with cat. 386 as the detection antibody.
- Immunogen for production of anti-collagen type I and III antibodies.
- Substrate for matrix metalloproteinase (MMP) activity and collagen-binding assays.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Sterile, gel-forming format: supplied sterile and salt-free, so it can be taken straight into cell culture without further clean-up — the practical requirement for 3-D gel and scaffold work.
- Reduced immunogenicity: telopeptide-depleted atelocollagen is the preferred form for implantation studies and for long-term culture where an immune reaction to telopeptides would confound the readout.
- Porcine source for translational models: porcine collagen is closely similar to human collagen and is widely used where a non-bovine, non-human matrix is required.
- Mixed I/III matrix: the type III minor component reproduces the composition of skin, vessel wall and granulation tissue more faithfully than pure type I, of value in wound-healing and fibrosis models.
- Bulk 25 mg format: sufficient for large gel volumes, scaffold casting and repeated coating campaigns.
