Description

Porcine collagen type I and III protein (atelocollagen). Sterile and lyophilized. Suitable for 3D-gel formation

Source

Porcine tissue

Purity

Porcine collagen type I > 90%, porcine collagen type I < 10%. Tested for sterility

Format

Lyophilized, salt-free

Purification

Collagen was extracted from washed dissected tissue with pepsin and dilute acetic acid. Collagen type I and III was purified by using differential salt precipitation

Applications

Coating material for cell culture studies (tested on human HepG2, primary rabbit corneal keratinocytes, rabbit corneal endothelial and epithelial cells, mouse embryonal fibroblasts, mouse peritoneal macrophages). Suitable for 3-D gel formation

Reconstitution

Acetic acid 10-50mM can be used for dissolving collagen to a concentration of 1-5 mg/ml overnight using gentle mixing at +4°C

Storage

Shipping at ambient temperature. Storage of lyophilized protein at -20°C. Storage of reconstituted protein 1 month at +4°C

RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE