Description

Collagen type I/III protein purified from porcine tendon (atelocollagen suitable for making 3D gel). Sterile and lyophilized

Source

Porcine tendons

Purity

Porcine collagen type I and III > 94% (SDS-PAGE)

Format

Lyophilized, salt-free

Purification

Collagen was extracted from porcine tendons with pepsin and acetic acid and then purified using salt precipitation

Sterility

Sterile

Applications

Cell culture (coating or 3D gel)

Reconstitution

Dissolve in 20 mM acetic acid at collagen concentration 5 mg/ml. Suspension should be stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension). Recommended concentration 1-5 mg/ml. After dissolution store 1 month at +4?.

Storage

Lyophilized protein: shipping at ambient temperature, long term storage at -20ºC or lower. Storage of reconstituted protein 1 month at +4ºC

RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE