Goat polyclonal antibody against human urokinase-type plasminogen activator (uPA / PLAU), affinity-purified on immobilised urokinase and cross-adsorbed against human plasma proteins, 0.1 mg. Binds human urokinase with no affinity to other plasma proteins. The goat-host member of YO’s uPA antibody family — pair with the mouse monoclonals cat. 335 (clone PGM2005) and cat. 381 (clone PGM2001), or with the neutralising rabbit polyclonal cat. 168, to build capture / detect uPA immunoassays.
SPECIFICATIONS
Catalogue number: 811 | Host: Goat | Target: Human
Target antigen: Urokinase-type plasminogen activator (uPA / PLAU)
Gene: PLAU (10q24.33) | UniProt: P00749
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds human urokinase; no affinity to human plasma proteins (cross-adsorbed).
Purification: Affinity-purified on immobilised urokinase, with cross-adsorption on immobilised human plasma proteins.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M NaCl, 1% dextran, 1% mannitol | Package size: 0.1 mg
STORAGE / RECONSTITUTION
Reconstitute with sterile pure water per the supplied protocol. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — detection of human uPA; use as the goat detection arm against a mouse-mAb capture (cat. 335 or cat. 381).
- Immunohistochemistry — uPA localisation in tumour and vascular tissue.
- Western blot — detection of uPA.
- Affinity purification — column ligand for uPA capture.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Cancer invasion / metastasis research — uPA in the tumour invasive front.
- Fibrinolysis and thrombosis research.
- uPA / PAI-1 biomarker immunoassay development.
- Goat-host detection arm for mouse-mAb-capture uPA sandwich assays.
