Description
Fibronectin heparin
binding 40 kDa fragment from chymotryptic digest of human plasma
fibronectin
Source
Human plasma. Negative for
HBsAg, HCV, HIV1 and HIV2
Purity
One major band at 40 kDa
and some lower bands on SDS-PAGE
Format
Lyophilized from solution
containing the product (1 mg/ml), sodium citrate 20 mM and sodium
chloride 150 mM, pH 7.4.
Purification
Affinity chromatography on
gelatine agarose and heparin agarose with subsequent gel filtration
Applications
Cell attachment and
integrin signaling assays
Promotes cytoskeleton
organization in integrin signaling assays
Reconstitution
Sterile 0.5 ml pure water.
Incubate 15 min at 37°C. Stir gently. Do not vortex.
Storage
Lyophilized protein:
shipping at ambient temperature, storage (1 year) at –20°C or
lower. Storage of reconstituted protein at -70°C for 6 months. Avoid
repeated freeze/thaw cycles
