Rabbit polyclonal antibody against the human IgM heavy (µ) chain, unconjugated IgG fraction, 1 mg. A high-titre, IgM-specific rabbit polyclonal — usable directly as a primary antibody in Western blot, IHC and immunofluorescence, as the capture reagent in IgM ELISA, as an affinity-column or bead ligand for IgM enrichment, and as starting material for in-house HRP / AP / biotin / fluorophore conjugation. Cited as the IgM-specific detection reagent in anti-SARS-CoV-2 Spike-RBD serology. The rabbit-host counterpart to the goat-host cat. 321.
SPECIFICATIONS
Catalogue number: 818 | Host: Rabbit | Target: Human
Target antigen: Human IgM heavy (µ) chain
Reactivity: Human IgM (µ-chain)
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Human IgM µ-chain specific.
Purification: Purified IgG fraction.
Format: Polyclonal, unconjugated, IgG fraction | Package size: 1 mg
STORAGE
Ships at ambient temperature. Store at -20 °C or lower; aliquot to avoid repeated freeze-thaw. Avoid microbial contamination.
APPLICATIONS
- Western blot — primary antibody for the human IgM heavy chain (high-titre rabbit polyclonal).
- IHC (FFPE) — plasma-cell and B-cell infiltrates.
- Immunofluorescence — human IgM B-cell subsets in tissue or cytospin.
- ELISA — detection of human IgM in serum and tissue digests, typically as the capture reagent.
- Affinity enrichment — couple to magnetic beads or affinity columns to enrich low-abundance human IgM.
- Custom conjugation — in-house HRP, AP, biotin or fluorophore labelling.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Infectious-disease serology — IgM-specific titres (e.g. anti-SARS-CoV-2 Spike-RBD IgM).
- Acute-infection / seroconversion research — IgM as the early-response isotype.
- Plasma-cell and B-cell pathology by IHC / IF.
- IgM affinity enrichment and depletion workflows.
REFERENCES
Notz Q, et al. (2020) Pro- and anti-inflammatory responses in severe COVID-19-induced acute respiratory distress syndrome — an observational pilot study. Front Immunol. 11:581338. doi:10.3389/fimmu.2020.581338. — a YO anti-human IgM (HRP-conjugated, 1:10 000) used as the isotype-specific detection reagent in an anti-SARS-CoV-2 Spike-RBD ELISA.
