Bovine collagen type II (atelocollagen), isolated from bovine knee-joint cartilage by pepsin treatment and extraction into dilute acetic acid, purified by differential salt precipitation and supplied lyophilised and salt-free, 1 mg. Type II collagen is the principal fibrillar collagen of hyaline cartilage, the nucleus pulposus and the vitreous humour, and is the defining autoantigen of collagen-induced arthritis. Use as a collagen type II standard, as immunogen, and as a cell-culture coating substrate — the small-pack format alongside cat. 826-30, the bovine counterpart to the human antigen cat. 210, and the matching standard for the anti-collagen type II antibodies cat. 214 and cat. 415.
SPECIFICATIONS
Catalogue number: 826-1 | Product: Collagen type II (bovine, atelocollagen)
Source: bovine knee joint
Purity: bovine collagen type II 95%; other bovine collagen types below 5%; non-collagen proteins below 0.5%
Format: lyophilised, salt-free
Purification: partial pepsin digestion under acidic conditions, extraction into dilute acetic acid and differential salt precipitation. Pepsin treatment removes the non-helical telopeptide regions (atelocollagen), reducing immunogenicity and improving solubility
Package size: 1 mg
STORAGE / RECONSTITUTION
Reconstitution: add 50 mM acetic acid to a collagen concentration of 1–5 mg/mL and mix vigorously overnight at +4 °C.
Shipped at ambient temperature.
Lyophilised protein: long-term storage 2 years at −20 °C or lower.
Reconstituted protein: store at +4 °C for up to 1 month.
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- Bovine collagen type II standard for ELISA and Western blot — pair with cat. 214 or cat. 415 as the detection antibody.
- Antigen for antibody production (immunogen).
- Coating material for cell-culture studies, in particular chondrocyte adhesion and phenotype-maintenance assays.
- Substrate for matrix metalloproteinase (MMP-13 / collagenase) activity assays.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Collagen-induced arthritis (CIA): bovine type II collagen is a standard immunogen for the CIA model, the most widely used preclinical model of rheumatoid arthritis.
- Cartilage and chondrocyte biology: coating with type II collagen helps chondrocytes retain their differentiated phenotype in monolayer, where type I substrates drive dedifferentiation.
- Osteoarthritis and cartilage-degradation assays: the physiological substrate for collagenase and MMP-13 cleavage studies and for neoepitope (C2C / CTX-II) assay development.
- Autoimmunity and oral tolerance: type II collagen is the antigen used in oral-tolerance and antigen-specific immunotherapy studies.
- Defined type-II standard: 95% type II with <5% other collagen types and <0.5% non-collagen protein, so it can serve as a calibrator where type specificity matters.
- Intervertebral disc and vitreous research: type II is the dominant collagen of the nucleus pulposus and vitreous humour.
