Bovine collagen type II (atelocollagen) isolated from bovine knee joint

Catalog Number 826-1

Amount 1 mg

Source

Bovine knee joint. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type II was purified by using differential salt precipitation

Purity

Bovine collagen type II 95%. Other bovine collagen types below 5%. Non-collagen proteins below 0.5%

Format

Lyophilized, salt-free

Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation

Applications

Bovine collagen (type II) standard.

Antigen for antibody production.

Coating material for cell culture studies.

Reconstitution

Add 50 mM acetic acid to collagen concentration 1-5 mg/ml. Mix vigorously overnight at +4ºC

Storage

Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20ºC or lower. Storage of reconstituted protein 1 month at +4ºC