collagen type II (atelocollagen) isolated from bovine knee joint
knee joint. Collagen was extracted from washed dissected tissue into
dilute acetic acid after pepsin treatment. Collagen type II was
purified by using differential salt precipitation
collagen type II 95%. Other bovine collagen types below 5%.
Non-collagen proteins below 0.5%
pepsin digestion in acidic conditions and differential salt
collagen (type II) standard.
for antibody production.
material for cell culture studies.
Add 50 mM
acetic acid to collagen concentration 1-5 mg/ml. Mix vigorously
overnight at +4ºC
protein: shipping at ambient temperature, long term storage (2 years)
at -20ºC or lower.
Storage of reconstituted protein 1 month at +4ºC