Rabbit polyclonal antibody against human collagen type VI, affinity-purified from rabbit antiserum on immobilised human collagen VI, 1 mL. Highly type-VI-selective — 100 % reactivity to collagen VI, < 3 % cross-reactivity to collagen I, II, III, IV and V, and < 0.5 % to human plasma proteins. For immunostaining of cryostat sections and of ECM in human cell culture, and for collagen-VI ELISA — pericellular microfibrillar-network, collagen-VI myopathy and ECM research. For broad collagen detection see the pan-collagen cat. 358.
SPECIFICATIONS
Catalogue number: 835 | Host: Rabbit | Target: Human
Target antigen: Human collagen type VI (beaded-microfibril collagen)
Gene: COL6A1 / COL6A2 / COL6A3 | UniProt: P12109, P12110, P12111
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Human collagen VI 100 %; cross-reactivity to human collagen I, II, III, IV and V < 3 %; human plasma proteins < 0.5 %.
Purification: Affinity-purified from rabbit antiserum on immobilised human collagen type VI.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M NaCl, 1% dextran, 1% mannitol | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — detection of human collagen type VI.
- Immunostaining (cryostat sections) — collagen-VI localisation in human tissue.
- Cell culture / ECM staining — collagen-VI microfibrillar networks deposited in human cell culture.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Collagen-VI myopathies — Bethlem myopathy and Ullrich congenital muscular dystrophy research.
- Muscle and adipose ECM biology — collagen VI as the pericellular microfibrillar network.
- Fibrosis and tumour-stroma collagen-VI deposition.
- ECM characterisation in tissue engineering and regenerative models.
