Rabbit polyclonal antibody against human thioredoxin (TXN), affinity-purified on immobilised recombinant human thioredoxin, 0.2 mL. Binds both native and denatured thioredoxin — so it performs in ELISA and in reducing Western blot alike — with no reactivity to human plasma proteins. For redox-biology, oxidative-stress and thioredoxin-system research.
SPECIFICATIONS
Catalogue number: 893 | Host: Rabbit | Target: Human
Target antigen: Recombinant human thioredoxin (TXN, ~12 kDa)
Gene: TXN (9q31.3) | UniProt: P10599
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds both native and denatured human thioredoxin; does not react with human plasma proteins.
Purification: Affinity chromatography on immobilised recombinant thioredoxin.
Format: Liquid, 1 mg/mL in 5 mM HEPES pH 7.0, 75 mM NaCl, 50 % glycerol | Package size: 0.2 mL
STORAGE
Ships at ambient temperature. Aliquot before freezing and avoid repeated freeze-thaw. Store at -20 °C or lower.
APPLICATIONS
- ELISA — thioredoxin detection (0.1–5 µg/mL).
- Western blot — 1–5 µg/mL; detects both native and denatured thioredoxin.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Redox biology and oxidative-stress research — the thioredoxin / thioredoxin-reductase system.
- Secreted thioredoxin as an inflammation and oxidative-stress biomarker.
- Cancer redox research — thioredoxin overexpression in tumours.
- Native and denatured reactivity — a single reagent across ELISA and reducing-WB workflows.