Heparin sodium salt, 250,000 units (1470 mg) — a highly purified, natural sulfated glycosaminoglycan derived from porcine intestinal mucosa. Its high negative charge density allows it to bind and activate antithrombin III, the natural inhibitor of thrombin and factor Xa, which is the basis of its anticoagulant activity. Supplied as a research-grade bulk powder for in-vitro coagulation and fibrinolysis assays, blood sampling and storage, thrombin inhibition, and general anticoagulation in biochemical and cell-based systems.
SPECIFICATIONS
Catalogue number: 904 | Source: Porcine intestinal mucosa
Composition: Heparin sodium salt, sulfated glycosaminoglycan
Appearance: White or off-white powder
Potency: ≥ 170 USP units/mg
Loss on drying: ≤ 10 %
Format: Lyophilised powder | Package size: 250,000 units (1470 mg)
Grade: Research use only (not for diagnostic, therapeutic or clinical use; not for use in humans or animals)
STORAGE / RECONSTITUTION
Reconstitution: dissolve in water or an appropriate aqueous buffer at the desired concentration; the powder is freely soluble.
Shipped at room temperature.
Store at −20 °C in a dry, cool place; stable for up to two years under these conditions.
Avoid repeated freeze-thaw cycles and exposure to light.
APPLICATIONS
- In-vitro coagulation and fibrinolysis assays — aPTT, anti-Xa and thrombin-generation systems.
- Anticoagulant for blood sampling, plasma preparation and sample storage.
- Inhibition of thrombin and factor Xa activity in biochemical and cell-based assays.
- Prevention of clotting in perfusion, flow-chamber and ex-vivo circuit models.
- Affinity-chromatography ligand and competitive eluent in heparin-binding protein purification.
- Cell-culture supplement — stabilisation of heparin-binding growth factors (FGF-2, VEGF, HB-EGF).
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Thrombosis and haemostasis research — antithrombin-III-dependent inhibition as a defined, dose-controllable anticoagulant.
- Heparin-binding proteome studies — competition and elution reagent for glycosaminoglycan-interaction mapping.
- Growth-factor signalling — obligate co-factor for FGF/FGFR complex formation in stem-cell and organoid culture.
- Extracellular-matrix and glycobiology — model highly sulfated GAG for charge-density and sulfation-pattern studies.
- Biomaterial and surface engineering — heparinised coatings for haemocompatible devices and scaffolds.
- Bulk pack size — 250,000 units supports large screening campaigns and process-scale method development.
