Collagen type I protein purified from bovine skin

Source

Bovine (calf) skin. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I was purified by using differential salt precipitation

Purity

Bovine collagen type I 90%. Other non-collagen proteins below 0.5%. Structure of native collagen was confirmed by ability to form microfibrils

Format

Lyophilized

Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation

Applications

Standard for immunological assays. Antigen for antibody production. Coating material for cell culture studies. May not be suitable for 3-D gel formation

Reconstitution

Use 0.1 M acetic acid, pH 3.0

Storage

Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20ºC or lower. Storage of reconstituted protein 1 month at +4ºC