Collagen type I protein purified from bovine skin

Source

Bovine (calf) skin. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I was purified by using differential salt precipitation

Purity

Bovine collagen type I 90%. Other non-collagen proteins below 0.5%. Structure of native collagen was confirmed by ability to form microfibrils

Format

Lyophilized

Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation

Applications

Type I collagen standard. Antigen for antibody production. Coating material for cell culture studies. May not be suitable for 3-D gel formation

Reconstitution

Use 0.1 M acetic acid, pH 3.0

Storage

Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20ºC or lower. Storage of reconstituted protein 1 month at +4ºC