Collagen type I
protein purified from bovine skin Source
Bovine (calf) skin.
Collagen was extracted from washed dissected tissue into dilute
acetic acid after pepsin treatment. Collagen type I was purified by
using differential salt precipitation
Purity
Bovine collagen type I
90%. Other non-collagen proteins below 0.5%. Structure of native
collagen was confirmed by ability to form microfibrils
Format
Lyophilized
Purification
Partial pepsin digestion
in acidic conditions and differential salt precipitation
Applications
Standard for immunological assays.
Antigen for antibody production. Coating material for cell culture
studies. May not be suitable for 3-D gel formation
Reconstitution
Use 0.1 M acetic acid, pH
3.0
Storage
Lyophilized protein:
shipping at ambient temperature, long term storage (2 years) at -20ºC
or lower. Storage of reconstituted protein 1 month at +4ºC |