Purified goat
collagen type I protein Source
Goat tissue. Collagen was
extracted from washed dissected tissue into dilute acetic acid after
pepsin treatment. Collagen type I was purified by using differential
salt precipitation
Purity
90% SDS-PAGE
Format
Lyophilized, salt-free
Purification
Partial pepsin digestion
in acidic conditions and differential salt precipitation
Applications
Standard for immunological assays. Antigen for antibody production.
Coating material for cell culture studies. Collagen microfibrils will
activate platelets causing aggregation. May not be suitable for 3-D gel formation
Reconstitution
Use 0.5 M acetic acid, pH
2.5. Dissolved collagen retains immunologic properties of native
collagen. Structure of native collagen confirmed by ability to form
microfibrils
Storage
Shipping at ambient
temperature. Storage 2 years at -20°C or lower |