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223 Collagen type I and III (porcine) 10 mg
Part Number 223
Price
Your Price: $173.00
On sale:
$99.00
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dispatched within 2 days
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1 $99.00
2 - 4 $89.10
5+ $79.20
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Description

Note: New users interested in porcine collagen type I/III should consider #785 instead, which is better than #223 (soluble in 20 mM acetic acid and suitable for 3D gels). This product #223 however is still available for a foreseeable future

Porcine collagen type I and III purified from pig's tissue

Source

Porcine tissue. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I and III was purified by using differential salt precipitation

Purity

Porcine collagens: type I – 45%, type III – 45%, type IV – 10%, type V below 1%. Non-collagen proteins below 0.5%

Format

Lyophilized, salt-free

Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation

Applications

Standard for immunological assays. Coating material for cell culture studies. May not be suitable for 3-D gel formation

Pig cell migration assay (1)

Reconstitution

Use 0.5 M acetic acid, pH 3.0. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils

Storage

Shipping at ambient temperature. Storage of lyophilized protein 2 years at -20°C. Storage of reconstituted protein 1 month at +4°C

Citations

1. O’Brien K, Bhatia A, Tsen F, Chen M, Wong AK, et al. (2014) Identification of the Critical Therapeutic Entity in Secreted Hsp90a That Promotes Wound Healing in Newly ReStandardized Healthy and Diabetic Pig Models. PLoS ONE 9(12): e113956. doi:10.1371/ journal.pone.0113956 PMID: 25464502

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