Human collagen type V purified from placenta
Source
Human placenta. Negative
for HBsAg, HCV, HIV1 and HIV2
Purity
Human collagen type V at
least 95%, other human collagens below 5%, non-collagen proteins
below 1%.
Format
Lyophilized.
Purification
Partial pepsin digestion
in acidic conditions and differential salt precipitation
Applications
Type V collagen
standard (1, 2, 3), Antigen for antibody production,
Coating material for cell culture studies
Detection of IgGs against disc ECM proteins by immunoblotting (1)
Reconstitution
Use 0.1 M acetic
acid, pH 3.0
Storage
Shipping at ambient
temperature. Storage 2 years at -20°C or lower
Citations
1. Capossela S, Schläfli P, Bertolo A, Janner T, Stadler BM, Pötzel T, Baur M, Stoyanov JV. Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins. Eur Cell Mater. 2014 Apr 4;27:251-63
2. Philips N, Chalensouk-Khaosaat J and Gonzalez S (2015) Stimulation of the Fibrillar Collagen and Heat Shock Proteins by Nicotinamide or Its Derivatives in Non-Irradiated or UVA Radiated Fibroblasts, and Direct Anti-Oxidant Activity of Nicotinamide Derivatives. Cosmetics 2015, 2(2), 146-161; doi:10.3390/cosmetics2020146
3. Philips N, Samuel P, Parakandi H, Gopal S, Siomyk H, Ministro A, Thompson T, Borkow G (2012) Beneficial Regulation of Fibrillar Collagens, Heat Shock Protein-47, Elastin Fiber Components, Transforming Growth Factor-ß 1, Vascular Endothelial Growth Factor and Oxidative Stress Effects by Copper in Dermal Fibroblasts. Connective Tissue Research 1-7
