Purified bovine immunoglobulin G (IgG), 1 mg — whole-molecule IgG isolated from normal bovine serum. Supplied as an immunochemical reference material: ELISA standard and calibrator for total bovine-IgG quantification, isotype / irrelevant-antibody control in immunostaining and flow cytometry, coating antigen for secondary-antibody titration, immunogen for raising anti-bovine IgG antisera, and blocking / adsorption reagent. Pairs with rabbit anti-bovine IgG + IgM + IgA cat. 531, and belongs to the purified immunoglobulin panel alongside human IgG cat. 697, rat IgG cat. 147, mouse IgG cat. 883, rabbit IgG cat. 700, bovine IgG cat. 404 and human IgA cat. 258.
SPECIFICATIONS
Catalogue number: 404 | Source: Normal bovine serum
Composition: Immunoglobulin G (IgG), whole molecule
Format: Purified immunoglobulin | Package size: 1 mg
STORAGE / RECONSTITUTION
Ships at ambient temperature. Store at −20 °C or lower for long-term storage. After reconstitution or thawing store at +4 °C for short-term use, or aliquot and freeze. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA standard / calibrator for total bovine-IgG quantification in serum, plasma and culture supernatant.
- Isotype and irrelevant-antibody control for bovine antibodies in IHC, ICC and flow cytometry.
- Coating antigen and positive control for titration and QC of anti-bovine IgG secondary conjugates — see rabbit anti-bovine IgG + IgM + IgA cat. 531.
- Immunogen for raising anti-bovine IgG antisera and secondary conjugates.
- Blocking / adsorption reagent to remove anti-bovine reactivity from multi-species assay systems.
- Reference protein for chromatography method development (Protein A / Protein G binding, IgG capture) — see also Protein A cat. 339.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Veterinary immunology — total bovine-IgG quantification in serum, milk and colostrum.
- Colostrum and passive-transfer research — calibrator for failure-of-passive-transfer assays.
- Cell-culture QC — reference IgG for foetal bovine serum characterisation and residual-IgG testing.
- Assay interference testing — bovine IgG as a defined blocking and cross-reactivity reagent.
- Chromatography development — bovine IgG as a weak Protein A binder for resin selectivity studies (cat. 339).
