Purified rat immunoglobulin G (IgG), 1 mg — whole-molecule IgG isolated from normal rat serum. Supplied as an immunochemical reference material: ELISA standard for total rat-IgG quantification, isotype / irrelevant-antibody control in immunostaining and flow cytometry, blocking and adsorption reagent, and immunogen for raising and titrating anti-rat IgG secondary antibodies. Part of the purified immunoglobulin panel alongside human IgG cat. 697, mouse IgG cat. 883, rabbit IgG cat. 700, bovine IgG cat. 404 and human IgA cat. 258.
SPECIFICATIONS
Catalogue number: 147 | Source: Normal rat serum
Composition: Immunoglobulin G (IgG), whole molecule
Format: Purified immunoglobulin | Package size: 1 mg
STORAGE / RECONSTITUTION
Ships at ambient temperature. Store at −20 °C or lower for long-term storage. After reconstitution or thawing store at +4 °C for short-term use, or aliquot and freeze. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA standard / calibrator for total rat-IgG quantification in serum, plasma, culture supernatant and hybridoma work.
- Isotype and irrelevant-antibody control for rat monoclonal antibodies in IHC, ICC and flow cytometry.
- Positive control and coating antigen for anti-rat IgG secondary-antibody titration and QC.
- Immunogen for raising anti-rat IgG antisera and secondary conjugates.
- Blocking / adsorption reagent to remove anti-rat reactivity from multi-species assay systems.
- Reference protein for chromatography method development (Protein A / Protein G binding, IgG capture) — see also Protein A cat. 339.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Rodent immunology — total-IgG quantification in rat disease, vaccination and toxicology models.
- Hybridoma and antibody development — isotype control and standard curve material for rat monoclonal workflows.
- Multi-species immunoassay design — cross-reactivity testing and adsorption of anti-rat reactivity.
- Secondary-antibody QC — coating antigen for validating anti-rat IgG conjugates.
- Purification method development — benchmark IgG for affinity-capture and process-chromatography studies.
