Recombinant Protein G
immobilized on 4% agarose (slurry) Format
Slurry containing 0.1 M
NaCl, 0.1 M sodium borate, 0.01 M sodium azide, pH 8.0
Ligand
Recombinant Protein G
Matrix
4% cross-linked agarose
Specificity
Binds Fc-fragment of most
subclasses of IgG of human, mouse, rat, rabbit, pig, goat and sheep.
Does not bind human IgM, IgA, IgD or IgE. Albumin-binding domain
deleted
Binding Capacity
Human IgG 10 mg per 1 ml
gel
Applications
Affinity chromatography of
mammalian immunoglobilins (whole molecules, Fc-fragments or immune
complexes). Selective extraction of immunoglobulin IgG from human
serum. Estimation of the IgG (antibody) content in immune sera and
astitic fluids
Recommended buffers
Binding buffer: 0.15 M
sodium chloride, 0.05 M sodium phosphate, pH 7.0
Elution buffer: 0.1 M
sodium chloride, 0.1 M sodium acetate, pH 2.5
Storage
Shipping at ambient
temperature. Storage at +4°C for 1 year. Do not freeze |