Monoclonal antibody (clone 3D12, IgG1 fraction) anti-human Apolipoprotein E (APOE)
Specificity
Human Apolipoprotein E
(all alleles). Reacts with LDL and VLDL. No cross-reactivity to other
human plasma proteins
Preparation
Ammonium sulphate
precipitation. DEAE-cellulose chromatography with gradient elution
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1%mannitol, pH 8
Applications
Western blotting, ELISA
(x1000), Immunohistochemistry (x100), IHC 1:25 (after deparaffinization, rehydration and treatment with pronase E ) (1)
Reconstitution
Use 1 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or
lower
References
1) Klaver CCW, Kliffen M, van Duijn CM, Hofman A, Cruts M, Grobbee DE, van Broeckhoven C, de Jong PTVM (1998) Genetic Association of Apolipoprotein E with Age-Related Macular Degeneration. Am. J. Hum. Genet. 63:200–206
2) Huter
O, Wolf HJ, Schnetzer A, Pfaller K (1997) Lipoprotein lipase, LDL
receptors and apo-lipoproteins in human fetal membranes at term.
Placenta 18(8):707-715
