Specificity
Sheep immunoglobulins IgG,
IgM, IgA. No cross-reactivity to other sheep plasma proteins.
Preparation
Purification by affinity
chromatography using antigen coupled to sorbent. Antibody
concentration was determined by extinction coefficient prior to
conjugation to fluorescein isothiocyanate (FITC). Chromatographic
separation of free fluorescein and protein aggregates.
Labeling four molecules FITC per antibody
Format
Antibody solution 1.2
mg/ml in the buffer (0.05 M sodium phosphate pH 7.5, 0.1 M NaCl, 50%
glycerol, 5 mg/ml BSA)
Applications and
recommended dilutions
Western blotting, ELISA,
Immunohistology (1:40), Cell staining, Cell sorting, Electron microscopy
Storage
Shipping at ambient
temperature. Storage at –20°C for 2 years |