Specificity

Sheep immunoglobulins IgG, IgM, IgA. No cross-reactivity to other sheep plasma proteins.

Preparation

Purification by affinity chromatography using antigen coupled to sorbent. Antibody concentration was determined by extinction coefficient prior to conjugation to fluorescein isothiocyanate (FITC). Chromatographic separation of free fluorescein and protein aggregates. Labeling four molecules FITC per antibody

Format

Antibody solution 1.2 mg/ml in the buffer (0.05 M sodium phosphate pH 7.5, 0.1 M NaCl, 50% glycerol, 5 mg/ml BSA)

Applications and recommended dilutions

Western blotting, ELISA, Immunohistology (1:40), Cell staining, Cell sorting, Electron microscopy

Storage

Shipping at ambient temperature. Storage at –20°C for 2 years