Specificity
Guinea pig
immunoglobulins. Affinity to human IgM, IgG, IgA below 1%. No
affinity to human serum albumin
Preparation
Purification by affinity
chromatography using immobilized guinea pig IgM, IgG, IgA.
Purification by gel filtration and anion-exchange chromatography to
remove protein aggregates and free peroxidase.
Format
Antibody solution 1.2
mg/ml in the buffer (0.1 M NaCl, 0.05 M sodium phosphate pH 7.5, 50%
glycerol, 5 mg/ml BSA)
Applications and
recommended dilutions
ELISA (x10000),
Immunohistochemistry, Blotting. Dilution should be adjusted to the appropriate task. Do not use azide together with HRP-conjugated products since azide strongly inhibits horseradish peroxidase (HRP)
Storage
Shipping at ambient
temperature. Storage at –20°C for 1 year |