Specificity

Guinea pig immunoglobulins. Affinity to human IgM, IgG, IgA below 1%. No affinity to human serum albumin

Preparation

Purification by affinity chromatography using immobilized guinea pig IgM, IgG, IgA. Purification by gel filtration and anion-exchange chromatography to remove protein aggregates and free peroxidase.

Format

Antibody solution 1.2 mg/ml in the buffer (0.1 M NaCl, 0.05 M sodium phosphate pH 7.5, 50% glycerol, 5 mg/ml BSA)

Applications and recommended dilutions

ELISA (x10000), Immunohistochemistry, Blotting. Dilution should be adjusted to the appropriate task. Do not use azide together with HRP-conjugated products since azide strongly inhibits horseradish peroxidase (HRP)

Storage

Shipping at ambient temperature. Storage at –20°C for 1 year