Monoclonal mouse antibody
anti-human IgG (IgG1, clone EFE-565), HRP conjugated Specificity
Human immunoglobulin IgG
(gamma chain). No cross-reaction to human IgA, IgE, IgM or other
human plasma proteins
Preparation
Precipitation with sodium
sulphate and DEAE-cellulose chromatography. Gel filtration and
ion-exchange chromatography to remove free peroxidase and
unconjugated antibody.
Format
Solution containing
antibody 1.2 mg/ml in buffer (0.05 M sodium phosphate, 0.1 M NaCl,
50% glycerol, 5 mg/ml BSA)
Applications and
recommended dilutions
Western blotting, ELISA,
Immunohistochemistry. Do not use azide together with HRP-conjugated products since azide strongly inhibits horseradish peroxidase (HRP)
Storage
Shipping at ambient
temperature. Storage 1 year at –20°C or lower |