Monoclonal mouse antibody anti-human IgG (IgG1, clone EFE-565), HRP conjugated

Specificity

Human immunoglobulin IgG (gamma chain). No cross-reaction to human IgA, IgE, IgM or other human plasma proteins

Preparation

Precipitation with sodium sulphate and DEAE-cellulose chromatography. Gel filtration and ion-exchange chromatography to remove free peroxidase and unconjugated antibody.

Format

Solution containing antibody 1.2 mg/ml in buffer (0.05 M sodium phosphate, 0.1 M NaCl, 50% glycerol, 5 mg/ml BSA)

Applications and recommended dilutions

Western blotting, ELISA, Immunohistochemistry. Do not use azide together with HRP-conjugated products since azide strongly inhibits horseradish peroxidase (HRP)

Storage

Shipping at ambient temperature. Storage 1 year at –20°C or lower