Polyclonal goat antibody anti-human IgM conjugated to horseradish peroxidase (HRP) (affinity purified and cross-adsorbed)

Specificity

Specific to human IgM (heavy chain). No cross-reactivity towards human IgG, IgA and other human plasma proteins

Preparation

Purification by affinity chromatography using immobilized human IgM with cross-adsorption against IgG and IgA. Purification by gel filtration and anion-exchange chromatography to remove protein aggregates and free peroxidase.

Format

Antibody solution 1.2 mg/ml in the buffer (0.05M sodium phosphate pH 7.5, 0.1M NaCl) containing 50% glycerol and 5 mg/ml bovine serum albumin

Applications and recommended dilutions

ELISA (x10000), Immunohistochemistry, Blotting. Dilution should be adjusted to the appropriate task. Do not use azide together with HRP-conjugated products since azide strongly inhibits horseradish peroxidase (HRP)

Storage

Shipping at ambient temperature. Storage for 1 year at -20°C or lower