Polyclonal goat antibody
anti-human IgM conjugated to horseradish peroxidase (HRP) (affinity
purified and cross-adsorbed) Specificity
Specific to human IgM
(heavy chain). No cross-reactivity towards human IgG, IgA and other
human plasma proteins
Preparation
Purification by affinity
chromatography using immobilized human IgM with cross-adsorption
against IgG and IgA. Purification by gel filtration and
anion-exchange chromatography to remove protein aggregates and free
peroxidase.
Format
Antibody solution 1.2
mg/ml in the buffer (0.05M sodium phosphate pH 7.5, 0.1M NaCl)
containing 50% glycerol and 5 mg/ml bovine serum albumin
Applications and
recommended dilutions
ELISA (x10000),
Immunohistochemistry, Blotting. Dilution should be adjusted to the
appropriate task. Do not use azide together with HRP-conjugated products since azide strongly inhibits horseradish peroxidase (HRP)
Storage
Shipping at ambient
temperature. Storage for 1 year at -20°C
or lower |