Polyclonal rabbit antibody
anti-chicken immunoglobulin IgY conjugated to horseradish peroxidase
(HRP) Specificity
Chicken immunoglobulin IgY
Preparation
Purification by affinity
chromatography using immobilized chicken IgY. Purification by gel
filtration and anion-exchange chromatography to remove protein
aggregates and free peroxidase
Format
Antibody solution 1.2
mg/ml in the buffer (0.1 M NaCl, 0.05M sodium phosphate pH 7.5, 50%
glycerol, 5 mg/ml BSA)
Applications and
recommended dilutions
ELISA (x10000),
Immunohistology, Blotting. Dilution should be adjusted to the appropriate task. Do not use azide together with HRP-conjugated products since azide strongly inhibits horseradish peroxidase (HRP)
Storage
Shipping at ambient
temperature. Storage at –20°C for 1 year |