Rabbit polyclonal antibody against human alpha-fetoprotein (AFP), affinity-purified and cross-adsorbed, 1 mL. Specific for human AFP with no reactivity to human albumin or immunoglobulins. A polyclonal detection reagent for human AFP immunoassay: pair with the monoclonal cat. 769 (mouse anti-human AFP) in sandwich ELISA and use cat. 858 (human AFP antigen) as the standard; the rabbit-host alternative to the goat polyclonal cat. 564 for multi-species assay design.
SPECIFICATIONS
Catalogue number: 605 | Host: Rabbit | Target: Human
Target antigen: Human alpha-fetoprotein (AFP)
Gene: AFP (4q13.3) | UniProt: P02771
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds human alpha-fetoprotein; no reactivity to human albumin or immunoglobulins.
Purification: Affinity chromatography on immobilised human alpha-fetoprotein, cross-adsorbed against human albumin and immunoglobulins.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M sodium chloride, 1% dextran, 1% mannitol, pH 8 | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — human AFP immunoassay. Pair with the monoclonal cat. 769; use cat. 858 (human AFP antigen) as the standard.
- Immunohistochemistry — detection of human AFP in tissue.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Oncology — AFP as a tumour marker in hepatocellular carcinoma and germ-cell tumours.
- Hepatology — AFP in liver regeneration, hepatitis and cirrhosis models.
- Cancer-diagnostics development — AFP in multi-marker diagnostic panels.
- Prenatal / developmental biology — AFP in fetal development and maternal screening research.
- Rabbit-host assay design — pairs with mouse and goat reagents without host conflict.
REFERENCES
Mikulits W, Reichl P (2015) Soluble Axl receptor tyrosine kinase in the diagnosis of cancer. WO2015140231 A1.
