Goat polyclonal antibody against human alpha-fetoprotein (AFP), affinity-purified and cross-adsorbed, 1 mg. Specific for human AFP with no reactivity to human albumin or immunoglobulins. The polyclonal partner for human AFP immunoassay: pair with the monoclonal cat. 769 (mouse anti-human AFP) in sandwich ELISA and use cat. 858 (human AFP antigen) as the standard; complements the rabbit polyclonal cat. 605.
SPECIFICATIONS
Catalogue number: 564 | Host: Goat | Target: Human
Target antigen: Human alpha-fetoprotein (AFP)
Gene: AFP (4q13.3) | UniProt: P02771
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds human alpha-fetoprotein; no reactivity to human albumin or immunoglobulins.
Purification: Affinity chromatography on immobilised human alpha-fetoprotein, cross-adsorbed against human albumin and immunoglobulins.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M sodium chloride, 1% dextran, 1% mannitol, pH 8 | Package size: 1 mg
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — human AFP immunoassay. Pair with the monoclonal cat. 769; use cat. 858 (human AFP antigen) as the standard.
- Immunohistochemistry — detection of human AFP in tissue.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Oncology — AFP as a tumour marker in hepatocellular carcinoma and germ-cell tumours.
- Hepatology — AFP in liver regeneration and disease.
- Prenatal / developmental biology — AFP in fetal development and maternal screening research.
- Immunoassay development — capture or detection arm for human AFP quantification.
- Biomarker validation — AFP measurement in serum and biological fluids.
