Rabbit polyclonal antibody against human Apolipoprotein A1 (ApoA1), the major protein of HDL, cross-adsorbed, 1 mL. Binds human ApoA1 with no affinity to other human plasma proteins. The polyclonal partner for human ApoA1 immunoassay: pair with the monoclonal cat. 851 (mouse anti-human ApoA1, clone 1C5) in sandwich ELISA and use cat. 789 (human ApoA1 antigen) as the standard.
SPECIFICATIONS
Catalogue number: 553 | Host: Rabbit | Target: Human
Target antigen: Human Apolipoprotein A1 (ApoA1), major HDL apolipoprotein
Gene: APOA1 (11q23.3) | UniProt: P02647
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds human Apolipoprotein A1; no affinity to other human plasma proteins.
Purification: Anion-exchange chromatography (DEAE-cellulose) with cross-adsorption against human plasma proteins.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M sodium chloride, 1% dextran, 1% mannitol, pH 8 | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C or lower. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — human ApoA1 immunoassay (dilution x1000). Pair with the monoclonal cat. 851; use cat. 789 (human ApoA1 antigen) as the standard.
- IHC (frozen sections) — human ApoA1 histochemistry (dilution x40).
- Dot blotting — detection of human ApoA1.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Lipoprotein metabolism — HDL / ApoA1 quantification in cardiovascular research.
- Atherosclerosis — ApoA1 and reverse cholesterol transport studies.
- Clinical biochemistry — ApoA1 as a cardiovascular risk marker.
- HDL structure / function — ApoA1 mapping in reconstituted and native particles.
- Amyloidosis research — ApoA1-derived amyloid detection.
