Rabbit polyclonal antibody against human Apolipoprotein B (ApoB-100), affinity-purified on immobilised highly purified ApoB, 0.5 mg. Reacts with human ApoB and intact LDL particles, with no reactivity to Apolipoprotein A1 or other human plasma proteins. The polyclonal arm of YO’s ApoB immunoassay family — pair with the mouse monoclonals cat. 182 (clone 4C11) or cat. 387 (clone 5F8) and use cat. 650 (human ApoB-100 antigen) as the standard.
SPECIFICATIONS
Catalogue number: 843 | Host: Rabbit | Target: Human
Target antigen: Apolipoprotein B (APOB; ApoB-100), including ApoB on intact LDL
Gene: APOB (2p24.1) | UniProt: P04114
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Reacts with human ApoB and LDL; no reactivity to Apolipoprotein A1 or other human plasma proteins.
Purification: Affinity chromatography on immobilised, highly purified Apolipoprotein B.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M NaCl, 1% dextran, 1% mannitol | Package size: 0.5 mg
STORAGE / RECONSTITUTION
Reconstitute with sterile pure water per the supplied protocol. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — detection of human Apolipoprotein B and LDL in plasma or serum (dilution 1:10 000).
- Sandwich ELISA — use as the polyclonal arm against a mouse-mAb capture (cat. 182 or cat. 387), with cat. 650 as the standard.
- Immuno-capture — detection of ApoB-containing lipoprotein particles.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Cardiovascular biomarker assays — ApoB quantification and the ApoB / ApoA-I ratio.
- Atherosclerosis and LDL biology.
- Familial hypercholesterolaemia and dyslipidaemia cohort studies.
- Lipoprotein immunoassay development.
