Mouse IgG1 monoclonal antibody against human Apolipoprotein B (APOB, ApoB-100 — the structural protein of LDL), clone 4C11, 0.5 mg. Epitope-mapped to the T3 (middle) peptide of ApoB-100 — recognises ApoB on intact LDL / VLDL / IDL particles. The epitope-distinct partner to cat. 387 (clone 5F8) for two-mAb ApoB sandwich ELISA (with cat. 650 human ApoB-100 antigen as the standard) and the capture antibody for immuno-affinity extraction of ApoB-containing lipoproteins. For ELISA, IHC / IF, Western blot and lipoprotein extraction.
SPECIFICATIONS
Catalogue number: 182 | Host: Mouse | Target: Human
Target antigen: Apolipoprotein B (APOB; ApoB-100, ~515 kDa; structural protein of LDL)
Gene: APOB (2p24.1) | UniProt: P04114
Clonality: Monoclonal | Clone: 4C11 | Isotype: Mouse IgG1 | Conjugate: Unconjugated
Epitope: T3 fragment (middle region) of human ApoB-100
Specificity: Recognises human ApoB on intact LDL / VLDL / IDL particles; no reactivity with other human serum proteins.
Purification: Sodium sulphate precipitation and anion-exchange chromatography (DEAE-cellulose)
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M NaCl, 1% dextran, 1% mannitol, pH 8 | Package size: 0.5 mg
STORAGE / RECONSTITUTION
Reconstitute with sterile pure water per the supplied protocol. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — detection / capture of human ApoB (LDL) in plasma or serum (dilution 1:1 000); the natural sandwich uses cat. 387 (clone 5F8, epitope-distinct) with cat. 650 (human ApoB-100 antigen) as the standard.
- Immuno-affinity extraction — couple to CNBr-Sepharose or Protein G beads for selective extraction of intact ApoB-containing lipoproteins (LDL / VLDL / IDL) from human plasma; elute under mild conditions.
- IHC / IF — starting dilution 1:100; ApoB-positive lipoprotein deposits in atherosclerotic plaque and hepatic tissue.
- Western blot — 1:1 000; detects intact ApoB-100 at ~515 kDa under reducing conditions.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- ApoB-particle immuno-extraction for proteomics — selective capture of intact LDL / VLDL / IDL.
- Cardiovascular biomarker assay development — epitope-defined middle-T3 capture.
- Atherosclerosis-plaque IHC — the middle-T3 epitope is accessible on intact subendothelial ApoB.
- Hepatic VLDL-secretion research — cellular ApoB in hepatocyte models.
- PCSK9-pathway pharmacology — immuno-extracted ApoB particles as substrate.
- Familial hypercholesterolaemia / dyslipidaemia cohort biomarker studies.
