Mouse monoclonal antibody against human Apolipoprotein B (APOB, ApoB-100 — the structural protein of LDL), clone 5F8, 1 mL. The epitope-distinct second mouse mAb in YO’s ApoB antibody family — pair with cat. 182 (clone 4C11) for epitope-distinct mouse-mAb / mouse-mAb sandwich ELISA on the same antigen, or with the rabbit polyclonal cat. 843. For ApoB ELISA, IHC, Western blot and immunoprecipitation.
SPECIFICATIONS
Catalogue number: 387 | Host: Mouse | Target: Human
Target antigen: Apolipoprotein B (APOB; ApoB-100, structural protein of LDL / VLDL)
Gene: APOB (2p24.1) | UniProt: P04114
Clonality: Monoclonal | Clone: 5F8 | Conjugate: Unconjugated
Specificity: Human ApoB-100; epitope-distinct from clone 4C11 (cat. 182), enabling two-mAb sandwich assays. Detects ApoB-100 (~515 kDa) and intestinal ApoB-48 (~250 kDa).
Format: Standard YO formulation | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute and handle per the supplied protocol. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — sandwich-ELISA capture or detection; pair with cat. 182 (clone 4C11, epitope-distinct) or the rabbit polyclonal cat. 843 on the opposite arm.
- IHC — ApoB-positive lipoprotein deposits in atherosclerotic plaque and hepatic VLDL-producing compartments.
- Western blot — detects intact ApoB-100 (~515 kDa) and intestinal ApoB-48 (~250 kDa).
- Immunoprecipitation — 1–5 µg antibody per 100–500 µL sample.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Two-mAb sandwich ELISA (5F8 / 4C11 epitope-distinct) — high-specificity ApoB quantification.
- Atherosclerosis-plaque IHC.
- Hepatic VLDL-secretion research (HepG2 / Huh7 supernatants).
- Cardiovascular biomarker assay development — ApoB / ApoA-I ratio.
- PCSK9-pathway pharmacology.
- Familial hypercholesterolaemia / dyslipidaemia cohort studies.
