Mouse monoclonal antibody against human Apolipoprotein B (ApoB / LDL), clone 12G10, IgG1, 1 mL. Binds the T4 fragment (residues 1—1297), the N-terminal region of human ApoB, with no reactivity to other human plasma proteins. Detects and captures ApoB-containing lipoproteins; use the human ApoB antigen cat. 650 as the ELISA standard and pair with the different-epitope ApoB-100 clones cat. 182 (4C11) or cat. 387 (5F8) for sandwich assays.
SPECIFICATIONS
Catalogue number: 447 | Host: Mouse | Target: Human
Target antigen: Human Apolipoprotein B (ApoB / LDL); T4 fragment, residues 1—1297 (N-terminal)
Gene: APOB (2p24.1) | UniProt: P04114
Clonality: Monoclonal | Clone: 12G10 | Isotype: IgG1 | Conjugate: Unconjugated
Specificity: Binds the N-terminal T4 fragment (res. 1—1297) of human ApoB; no reactivity to other human plasma proteins.
Purification: Sodium sulphate precipitation and DEAE-cellulose anion-exchange chromatography with gradient elution
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M sodium chloride, 1% dextran, 1% mannitol, pH 8 | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C for up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA (dilution x1000) — detection of Apolipoprotein B (LDL) in human plasma or serum; use antigen cat. 650 as the standard.
- Immunohistology (dilution x100) — ApoB localisation in tissue sections.
- Lipoprotein extraction — immunoaffinity isolation of ApoB-containing lipoproteins from human plasma.
- Sandwich ELISA — N-terminal capture; pair with different-epitope clones cat. 182 (4C11) or cat. 387 (5F8).
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Cardiovascular risk — ApoB is the primary structural protein of atherogenic LDL and a key CVD biomarker.
- Atherosclerosis research — ApoB-lipoprotein deposition in plaque and vessel wall.
- Lipoprotein metabolism — quantification and isolation of LDL / VLDL particles.
- Dyslipidaemia & metabolic syndrome — ApoB profiling in clinical research cohorts.
- N-terminal epitope studies — defined T4-fragment binding for structure-function work.
