Monoclonal mouse (murine)
antibody (IgG1 fraction) anti-human Apolipoprotein B, clone 12G10
Specificity
Specific against T4
fragment (res. 1-1297), the N-terminal thrombolytic peptide of human
Apolipoprotein B (LDL). No reactivity to other human plasma proteins.
Preparation
Precipitation by sodium
sulphate and anion-exchange chromatography (DEAE-cellulose) with
gradient elution
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1%mannitol, pH 8
Applications and
recommended dilutions
Detection of
Apolipoprotein B (LDL) in human plasma or serum
Extraction of Apo-B
containing lipoproteins from human plasma
ELISA (x1000),
Immunohistology (x100)
Reconstitution
Use 1 ml sterile pure
water, aliquote and store at -20°C or lower.
Storage
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or
lower.
