Mouse IgG1 monoclonal antibody against human Apolipoprotein E (APOE, 34 kDa), clone 3D12, 1 mL. Pan-isoform — recognises all three common ApoE isoforms (e2, e3, e4) with no cross-reactivity to other human plasma proteins. The research-grade reagent for ApoE detection in atherosclerotic plaques, Alzheimer’s-disease amyloid deposition, cerebral amyloid angiopathy, astrocyte / microglial biology and lipoprotein-particle (VLDL / IDL / HDL / chylomicron-remnant) characterisation. For sandwich ELISA, pair with the rabbit polyclonal cat. 359. Validated for IHC, Western blot and ELISA.
SPECIFICATIONS
Catalogue number: 141 | Host: Mouse | Target: Human
Target antigen: Apolipoprotein E (ApoE / APOE, 34 kDa)
Gene: APOE (19q13.32) | UniProt: P02649
Clonality: Monoclonal | Clone: 3D12 | Isotype: Mouse IgG1 | Conjugate: Unconjugated
Isoform coverage: All three common alleles (e2, e3, e4) — pan-isoform
Specificity: Specific for human ApoE; no cross-reactivity with other human plasma proteins. Reacts with ApoE-positive VLDL / IDL / HDL.
Format: Purified IgG1 fraction | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- IHC (FFPE) — HIER, citrate pH 6.0; 1:50 to 1:200. Atherosclerotic-plaque ApoE deposition and Alzheimer’s-disease amyloid plaques.
- IHC (frozen sections) — 1:100 to 1:400; no antigen retrieval typically required.
- IF — 1:50 to 1:200; co-localisation studies with amyloid-ß and ApoJ / clusterin.
- Western blot — 1:500 to 1:2 000; detects the 34 kDa ApoE monomer (may also run as a dimer).
- ELISA (capture or detection) — pan-isoform capture; for sandwich ELISA pair with cat. 359 (rabbit anti-ApoE polyclonal).
- Functional / lipoprotein-particle analysis — immunoprecipitation of ApoE-containing lipoprotein particles, lipoprotein-gradient fractions, astrocyte- / microglia-secreted ApoE and HDL reconstitution.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Alzheimer’s-disease neuropathology — pan-isoform total-ApoE quantification in brain, CSF and tissue sections across mixed-genotype cohorts.
- Atherosclerosis / vascular research — ApoE plaque and lipoprotein deposition.
- Lipoprotein biology and metabolism — ApoE-particle identification.
- iPSC-derived astrocyte and microglia models — secreted-ApoE detection.
REFERENCES
1) Klaver CCW, Kliffen M, van Duijn CM, Hofman A, Cruts M, Grobbee DE, van Broeckhoven C, de Jong PTVM (1998) Genetic association of apolipoprotein E with age-related macular degeneration. Am J Hum Genet. 63:200-206.
2) Huter O, Wolf HJ, Schnetzer A, Pfaller K (1997) Lipoprotein lipase, LDL receptors and apolipoproteins in human fetal membranes at term. Placenta. 18(8):707-715.
