Rabbit polyclonal antibody against human Apolipoprotein E (APOE, 34 kDa), cross-adsorbed against other human plasma proteins, 1 mL. Pan-isoform — binds human ApoE (e2 / e3 / e4) with no affinity to other plasma proteins. The polyclonal detection partner to the mouse mAb cat. 141 (clone 3D12) for ApoE sandwich ELISA, and a reagent for ApoE detection in atherosclerotic plaques, Alzheimer’s amyloid deposition and lipoprotein-particle characterisation. Validated for ELISA, frozen-section IHC and dot blot.
SPECIFICATIONS
Catalogue number: 359 | Host: Rabbit | Target: Human
Target antigen: Apolipoprotein E (ApoE / APOE, 34 kDa)
Gene: APOE (19q13.32) | UniProt: P02649
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds human ApoE (all isoforms); no affinity to other human plasma proteins (cross-adsorbed).
Purification: Anion-exchange chromatography (DEAE-cellulose) with cross-adsorption against human plasma proteins.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M NaCl, 1% dextran, 1% mannitol | Package size: 1 mL (reconstitute in 1 mL sterile water)
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water; aliquot and freeze at -20 °C or lower. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — human ApoE detection (1:1 000); for sandwich ELISA pair with the mouse mAb cat. 141 (clone 3D12).
- IHC (frozen sections) — ApoE deposition (1:30); atherosclerotic plaque and Alzheimer’s amyloid.
- Dot blot — ApoE detection.
- Cell culture — astrocyte- / microglia-secreted ApoE and lipoprotein-particle ApoE.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Alzheimer’s-disease and neurodegeneration research — ApoE in amyloid deposition.
- Atherosclerosis / vascular research — plaque ApoE.
- Lipoprotein biology and metabolism.
- Astrocyte / microglia ApoE-secretion models.
