Monoclonal antibody
anti-human CD31 (Platelet GPIIa', PECAM1) (clone VM64, IgG2b kappa
fraction)
Specificity
The monoclonal antibody is
directed against the CD31 antigen, a 140 kD protein, which is
expressed on human platelets and endothilial cells and is designated
as PECAM-1 (platelet-endothelial cell adhesion molecule). The
antibody reacts with platelets, monocytes/macrophages, granulocytes
and B-cells. It stains the cell boundaries of endothelial cells with
a staining pattern identical to that of silverstaining. Furthermore
in histology it stains weakly histocytes and glomeruli
Preparation
Ammonium sulphate
precipitation and DEAE-cellulose chromatography with gradient elution
Format
Purified and lyophilized. The lyophilized antibody (0.2 mg) contains mannitol, dextran, and salts
Applications
Functional studies on
cell-cell interactions. Studies on integrity of endothelial cell
monolayers. The antibody can be used for immunoblotting under non
reducing conditions. The antibody is useful for indirect
immunofluorescence staining with analysis by cytofluorometry or
fluorescence microscopy and for immunohistochemistry.
Endothelial Cell Marker: CD31 is primarily used as an endothelial cell marker. It helps identify and characterize endothelial cells in various tissues.
Angiogenesis Studies: CD31 staining is frequently used in research to study angiogenesis (the formation of new blood vessels), which plays a role in processes like wound healing, tumor growth, and cardiovascular diseases.
Immunohistochemistry (IHC): In pathology and research, CD31 is used in IHC staining to identify endothelial cells in tissue sections. This can be crucial for distinguishing vascular neoplasms from non-vascular tumors.
Tumor Vasculature Analysis: In cancer research, CD31 staining can be used to analyze the vasculature within tumors, providing insights into tumor angiogenesis and potential therapeutic interventions.
Leukocyte Transmigration: CD31 plays a role in the transmigration of leukocytes through the endothelial cell junctions. As such, antibodies against CD31 are used in studies related to inflammation and immune response.
Flow Cytometry: It's used in flow cytometry to differentiate and analyze endothelial cells from other cell types in a mixed cell population.
Cell Sorting: Used in conjunction with cell sorting technologies to isolate populations of endothelial cells for various research applications.
Reconstitution
Use 1 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or
lower
