Monoclonal antibody
anti-human CD31 (Platelet GPIIa', PECAM1) (clone VM64, IgG2b kappa
fraction)
Specificity
The monoclonal antibody is
directed against the CD31 antigen, a 140 kD protein, which is
expressed on human platelets and endothilial cells and is designated
as PECAM-1 (platelet-endothelial cell adhesion molecule). The
antibody reacts with platelets, monocytes/macrophages, granulocytes
and B-cells. It stains the cell boundaries of endothelial cells with
a staining pattern identical to that of silverstaining. Furthermore
in histology it stains weakly histocytes and glomeruli
Preparation
Ammonium sulphate
precipitation and DEAE-cellulose chromatography with gradient elution
Format
Lyophilized antibody (0.2 mg). Contains mannitol, dextran, salts
Applications
Functional studies on
cell-cell interactions. Studies on integrity of endothelial cell
monolayers. The antibody can be used for immunoblotting under non
reducing conditions. The antibody is useful for indirect
immunofluorescence staining with analysis by cytofluorometry or
fluorescence microscopy and for immunohistochemistry.
Reconstitution
Use 1 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or
lower
