Monoclonal antibody anti-human CD41a (IgG1 kappa, clone VM16a) specifically targets the Platelets GPIIb/IIIa complex, also known as alphaIIb/beta3-integrin
Specificity
Specific to platelet
GPIIb/IIIa complex (alphaIIb/beta3-integrin). The antibody does not
bind dissociated GPIIb or GPIIIa, platelets with Glanzmann
thrombasthenia or vitronectin receptor.
Preparation
PEG6000 precipitation and
DEAE-cellulose chromatography with gradient elution
Format
Purified and lyophilized. The lyophilized antibody (0.2 mg) contains mannitol, dextran, and salts
Applications and
recommended dilutions
Flow Cytometry: This antibody can be used in flow cytometry to identify and characterize platelets and megakaryocytes based on the expression of CD41/Integrin alpha 2b.
Immunohistochemistry (IHC): It can be used in IHC to detect and visualize CD41/Integrin alpha 2b expressing cells in tissue sections, helping to identify platelets and megakaryocytes within tissues.
Western Blotting: The antibody might be used in Western blotting to detect CD41/Integrin alpha 2b in protein samples, helping researchers to understand its expression levels in different conditions or tissues.
Platelet Aggregation Studies: CD41 is a part of the complex that mediates platelet aggregation. Therefore, this antibody can be used in studies investigating the mechanisms underlying platelet aggregation.
Hematologic Disease Research: This antibody can be utilized in research to study hematologic diseases and disorders, including thrombocytopenia, leukemia, and other platelet disorders, as CD41 is a key marker for platelets and megakaryocytes.
Thrombosis Research: It can be used in thrombosis research, where understanding the role of platelets and their integrins is crucial.
Cell Adhesion Studies: CD41/Integrin alpha 2b is involved in cell adhesion processes, and thus the antibody can be used in studies investigating cell-cell and cell-matrix interactions.
Immunoprecipitation: This technique might utilize the antibody to isolate CD41/Integrin alpha 2b along with its binding partners from a mixture of proteins, facilitating the study of its interactions and functions at the molecular level.
Reconstitution
Use 1 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or
lower
