Monoclonal antibody
anti-human CD42b (Platelet GPIb) (clone VM16d, IgG1 kappa fraction)
Specificity
The monoclonal antibody is
directed against the CD42b antigen, platelet glycoprotein GPIb, that
serves as a receptor for von Willebrand factor and as a high affinity
thrombin receptor. The antigen is expressed on human platelets and
megakaryocytes. The antigen is absent or present in very low levels
on platelets of patients with the Bernard-Soulier syndrome. VM16d
inhibits low conc. thrombin (<0.1 U/ml) induced platelet
aggregation and decreases thrombin binding to platelets. F(ab')2 and
F(ab') fragments are equally effective as whole intact antibodies.
the VM16d-epitope is located within the 45 kDa N-terminal domain of
GPIb alpha- chain. VM16d does not effect platelet interaction with
von Willebrand factor, leading to the conclusion that the antibody
reacts with or nearby the thrombin receptor site
Preparation
Ammonium sulphate
precipitation and DEAE-cellulose chromatography with gradient elution
Format
Lyophilized antibody (0.2 mg). Contains mannitol, dextran, salts
Applications
Analysis of platelet
aggregation and detection of GPIb expression. The antibody is also
useful for functional studies
Reconstitution
Use 1 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or
lower
Citation
Qi Y et al. Novel antibodies against GPIb alpha inhibit pulmonary metastasis by affecting vWF-GPIb alpha interaction. Corpus ID: 108285710
