Monoclonal antibody
anti-human CD42b (Platelet GPIb) (clone VM16d, IgG1 kappa fraction)
Specificity
The monoclonal antibody is
directed against the CD42b antigen, platelet glycoprotein GPIb, that
serves as a receptor for von Willebrand factor and as a high affinity
thrombin receptor. The antigen is expressed on human platelets and
megakaryocytes. The antigen is absent or present in very low levels
on platelets of patients with the Bernard-Soulier syndrome. VM16d
inhibits low conc. thrombin (<0.1 U/ml) induced platelet
aggregation and decreases thrombin binding to platelets. F(ab')2 and
F(ab') fragments are equally effective as whole intact antibodies.
the VM16d-epitope is located within the 45 kDa N-terminal domain of
GPIb alpha- chain. VM16d does not effect platelet interaction with
von Willebrand factor, leading to the conclusion that the antibody
reacts with or nearby the thrombin receptor site
Preparation
Ammonium sulphate
precipitation and DEAE-cellulose chromatography with gradient elution
Format
Lyophilized antibody (0.2 mg). Contains mannitol, dextran, salts
Applications
Flow Cytometry: The most common use of anti-CD42b antibodies is in flow cytometry to identify and quantify platelets or to differentiate between various stages of megakaryocyte (platelet precursor) differentiation.
Platelet Function Analysis: The antibody can be used in assays designed to study the function of platelets, particularly those assays that evaluate the role of the GPIb-IX-V complex in platelet adhesion and aggregation.
Immunoprecipitation and Western Blotting: The antibody can pull down CD42b from cellular lysates, allowing researchers to study its interactions with other proteins or post-translational modifications.
Immunohistochemistry and Immunofluorescence: The antibody can be used to stain tissue sections or cells to visualize the location and expression pattern of CD42b.
Reconstitution
Use 1 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or
lower
Citation
