anti-human CD42b (Platelet GPIb) (clone VM16d, IgG1 kappa fraction)
The monoclonal antibody is
directed against the CD42b antigen, platelet glycoprotein GPIb, that
serves as a receptor for von Willebrand factor and as a high affinity
thrombin receptor. The antigen is expressed on human platelets and
megakaryocytes. The antigen is absent or present in very low levels
on platelets of patients with the Bernard-Soulier syndrome. VM16d
inhibits low conc. thrombin (<0.1 U/ml) induced platelet
aggregation and decreases thrombin binding to platelets. F(ab')2 and
F(ab') fragments are equally effective as whole intact antibodies.
the VM16d-epitope is located within the 45 kDa N-terminal domain of
GPIb alpha- chain. VM16d does not effect platelet interaction with
von Willebrand factor, leading to the conclusion that the antibody
reacts with or nearby the thrombin receptor site
precipitation and DEAE-cellulose chromatography with gradient elution
Lyophilized antibody (0.2 mg). Contains mannitol, dextran, salts
Flow Cytometry: The most common use of anti-CD42b antibodies is in flow cytometry to identify and quantify platelets or to differentiate between various stages of megakaryocyte (platelet precursor) differentiation.
Platelet Function Analysis: The antibody can be used in assays designed to study the function of platelets, particularly those assays that evaluate the role of the GPIb-IX-V complex in platelet adhesion and aggregation.
Immunoprecipitation and Western Blotting: The antibody can pull down CD42b from cellular lysates, allowing researchers to study its interactions with other proteins or post-translational modifications.
Immunohistochemistry and Immunofluorescence: The antibody can be used to stain tissue sections or cells to visualize the location and expression pattern of CD42b.
Use 1 ml sterile pure
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or