Monoclonal antibody anti-human CD61
(integrin beta 3, ß-integrin, Platelet GPIIIa, VNR ß-chain 3)
(IgG1 kappa, clone CRC54)
Specificity
Reacts with platelet
glycoprotein gpIIIa/VNR ß chain. This glycoprotein is the common ß
chain (ß3 Integrin) of the cytoadhesins, gpIIbIIIa and VNR. Reacts
with platelets and megakaryocytes. The antibody reacts preferentially
with the activated/occupied form of platelet GPIIb-IIIa
(ligand-induced binding site or LIBS epitope) and
dissociated form of GPIIIa. CRC54-epitope is located within the
N-terminal region of GPIIIa (first 100 aminoacids). CRC54 does not
react with platelets of patients with Glanzmann thrombastenia.
Preparation
Ammonium sulphate
precipitation and DEAE-cellulose chromatography with gradient elution
Format
Lyophilized antibody (0.2 mg). Contains mannitol, dextran, salts
Applications and
recommended dilutions
Immunoassay,
Immunofluorescence, FACS, IHC (frozen)
Antibody can be used for:
-Determination of
thrombocytes in blood.
-Identification of
megakaryocytes and -blasts in tissue.
-Immunoblotting under
reduced and nonreduced conditions.
-Detection of
conformational changes of GPIIb-IIIa on activated platelets.
Reconstitution
Use 1 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or
lower
