Monoclonal antibody
anti-human CD62 (P-selectin, GMP 140, PADGEM) (clone CRC81, IgG1
kappa fraction)
Specificity
Reacts with CD62 antigen
(P-selectin, integral membrane sialoglycoprotein 140 kDa, GMP-140,
granule membrane protein 140, PADGEM), which is expressed in platelet
alpha granules that are translocated to the platelet surface upon
activation with strong agonists. A similar protein, present in
endothelial cell Weibel-Palade bodies, is transiently expressed on
the surface of activated endothelial cells and functions as adhesion
molecule for granulocytes. The antibody does not interfere with the
functional activity of CD62 as an adhesion receptor for neutrophils
Preparation
Ammonium sulphate
precipitation and DEAE-cellulose chromatography with gradient elution
Format
Lyophilized antibody (0.2 mg). Contains mannitol, dextran, salts
Applications
Flow Cytometry: To identify and quantify P-selectin expression on the surface of activated endothelial cells or platelets. This helps in studying cell activation under various conditions or treatments.
Immunohistochemistry: To visualize the localization of P-selectin in tissue samples, potentially aiding in the diagnosis or understanding of various conditions such as inflammation or vascular diseases.
Immunoprecipitation: To pull down P-selectin from cell lysates for further molecular analyses or protein-protein interaction studies.
Western Blotting: To detect P-selectin in protein samples, helping to quantify its expression in various conditions or treatments.
Inhibition Studies: The antibody might be used to block the function of P-selectin, allowing researchers to study the effects of P-selectin inhibition in various in vitro and in vivo models. This can be particularly useful in understanding diseases like atherosclerosis, thrombosis, or other inflammatory conditions.
ELISA (Enzyme-Linked Immunosorbent Assay): For detecting or quantifying soluble P-selectin levels in biological fluids.
Cell Adhesion Assays: To study the role of P-selectin in mediating cell-cell interactions, particularly between leukocytes and endothelial cells or platelets during inflammation.
Therapeutic Targeting: While the specific clone might not be directly used for therapeutic purposes, understanding its binding efficacy and specificity can be critical in the development of therapeutic antibodies targeting P-selectin in various diseases.
Reconstitution
Use 1 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or
lower
