Monoclonal antibody
anti-human CD62 (P-selectin, GMP 140, PADGEM) (clone CRC81, IgG1
kappa fraction)
Specificity
Reacts with CD62 antigen
(P-selectin, integral membrane sialoglycoprotein 140 kDa, GMP-140,
granule membrane protein 140, PADGEM), which is expressed in platelet
alpha granules that are translocated to the platelet surface upon
activation with strong agonists. A similar protein, present in
endothelial cell Weibel-Palade bodies, is transiently expressed on
the surface of activated endothelial cells and functions as adhesion
molecule for granulocytes. The antibody does not interfere with the
functional activity of CD62 as an adhesion receptor for neutrophils
Preparation
Ammonium sulphate
precipitation and DEAE-cellulose chromatography with gradient elution
Format
Lyophilized antibody (0.2 mg). Contains mannitol, dextran, salts
Applications
Immunoflurescence
(indirect). Detection of activated platelets. Recommended dilution
x100
Reconstitution
Use 1 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or
lower
