Mouse monoclonal antibody against human CD62P (P-selectin; GMP-140 / PADGEM), clone CRC81, 0.2 mg. Reacts with the 140 kDa integral-membrane sialoglycoprotein stored in platelet a-granules and endothelial Weibel-Palade bodies and surface-expressed on activation. For platelet and endothelial activation studies — flow cytometry, IHC, immunoprecipitation, Western blot, soluble-P-selectin ELISA, function-blocking and cell-adhesion assays. Complements the platelet markers cat. 751 (aIIbß3), cat. 136 (CD31) and cat. 192 (CD36).
SPECIFICATIONS
Catalogue number: 819 | Host: Mouse | Target: Human
Target antigen: CD62P / P-selectin (GMP-140, PADGEM) — 140 kDa integral-membrane sialoglycoprotein
Gene: SELP (1q24.2) | UniProt: P16109
Clonality: Monoclonal | Clone: CRC81 | Conjugate: Unconjugated
Specificity: Reacts with human CD62P (P-selectin).
Purification: Ammonium sulphate precipitation and DEAE-cellulose chromatography.
Format: Lyophilised (mannitol, dextran, salts) | Package size: 0.2 mg
STORAGE / RECONSTITUTION
Reconstitute with sterile pure water per the supplied protocol. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- Flow cytometry — identify and quantify P-selectin surface expression on activated platelets and endothelial cells.
- Immunohistochemistry — P-selectin localisation in tissue.
- Immunoprecipitation — pull down P-selectin from cell lysates.
- Western blot — P-selectin detection and quantification.
- ELISA — detection / quantification of soluble P-selectin.
- Function-blocking and cell-adhesion assays — block P-selectin-mediated cell–cell interactions (e.g. leukocyte rolling).
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Platelet-activation research — P-selectin as the canonical a-granule degranulation marker.
- Endothelial activation and Weibel-Palade body exocytosis.
- Leukocyte rolling and inflammation — P-selectin / PSGL-1 adhesion.
- Thrombo-inflammation and soluble-P-selectin biomarker studies.
