Polyclonal rabbit antibody anti-human collagen type I and III (COL1A1, COL3A1)

Specificity

Binds human collagen type I and III. Cross-reactivity to other human collagens as measured by ELISA type IV below 3%, type V below 2%.

Preparation

Affinity purification on immobilized human collagen type I and III with cross-absorption to immobilised human blood plasma proteins and human collagen type II, IV and V.

Format

Purified and lyophilized. Contains mannitol, dextran and salts

Applications and recommended dilutions

Detection of human collagen in ELISA (x4000), Immunohistochemistry (frozen sections) (x30), Immunostaining of ECM in human cell cultures. Not suitable for denaturing and dissociating conditions.

Reconstitution

Use 0.5 ml sterile pure water

Storage

Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower