Monoclonal mouse antibody (clone MC4-HA, IgM fraction) anti-human Collagen type IV

Specificity

Binds Human Collagen type IV in native and heat-denatured state. No cross-reactivity to human collagen I, II, III and V.

Preparation

PEG-6000 precipitation and purification by gel chromatography

Format

Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1%mannitol, pH 8

Applications and recommended dilutions

ELISA (x1000), Immunohistochemistry (x20). For immunohistochemistry on frozen sections fixation with acetone for 5 min at -20C is recommended.

Reconstitution

Use 1 ml sterile pure water

Storage

Storage of lyophilized antibodies 2 years -20°C or lower