Rabbit polyclonal antibody against native human collagen type I (COL1A1 / COL1A2), cross-absorbed against other human collagens, 1 mL. Type-I-specific — no reactivity to human collagen II, III, IV or V. The native-conformation collagen-I reagent (companion to cat. 287) for collagen-I ELISA — pair with the mouse mAb cat. 393 or use cat. 288 (human collagen I antigen) as the standard — and frozen-section IHC of dermal, bone, tendon and fibrotic tissue. Not suitable for denaturing conditions.
SPECIFICATIONS
Catalogue number: 323 | Host: Rabbit | Target: Human
Target antigen: Native human collagen type I (COL1A1 / COL1A2), triple-helical fold
Gene: COL1A1 (17q21.33), COL1A2 (7q21.3) | UniProt: P02452, P08123
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Specific for human collagen I; no reactivity to human collagen II, III, IV or V. Native-conformation — not suitable for denaturing conditions.
Purification: Sodium sulphate precipitation, anion-exchange chromatography (DEAE-cellulose) and cross-absorption against other human collagens.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M NaCl, 1% dextran, 1% mannitol | Package size: 1 mL (reconstitute in 1 mL sterile water)
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water; aliquot and store at -20 °C or lower. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — detection of human collagen I; use cat. 288 (human collagen I antigen) as the standard, or pair with the mouse mAb cat. 393.
- IHC (frozen sections) — collagen-I histochemistry (starting dilution 1:10 to 1:20) of dermal, bone, tendon and fibrotic tissue.
- Cell culture / ECM staining — collagen-I matrix deposited by fibroblasts and osteoblasts.
- Note: native-conformation antibody — not suitable for denaturing conditions.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Fibrosis research — collagen-I deposition in dermal, hepatic, pulmonary, renal and cardiac fibrosis.
- Bone and tendon biology — type-I collagen in musculoskeletal tissue.
- Tumour stroma — collagen-I by cancer-associated fibroblasts.
- Collagen-scaffold / tissue-engineering QC.
