Rabbit affinity-purified polyclonal antibody against rat collagen type I, 0.5 mL. Cross-absorbed against rat collagen type III, so it reads type-I specifically within the rat fibrillar-collagen pair — the type-I-selective complement to cat. 352 (rabbit anti-rat collagen I+III). The reagent for rat collagen-I sandwich ELISA, frozen-section collagen-I histochemistry, and immunostaining of collagen-I ECM in rat cell culture. Pairs with cat. 351 (rat collagen type I antigen) as the natural ELISA standard, and with cat. 864 (rabbit anti-rat collagen III) for side-by-side type-I-vs-III fibril mapping.
SPECIFICATIONS
Catalogue number: 376 | Host: Rabbit | Target: Rat
Target antigen: Rat collagen type I
Gene family: COL1 (COL1A1 / COL1A2)
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds rat collagen type I; ~50% cross-reactivity to denatured collagen I; cross-absorbed against rat collagen type III
Purification: Affinity-purified on immobilised collagen type I, cross-absorbed on immobilised rat collagen type III
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M sodium chloride, 1% dextran, 1% mannitol, pH 8 | Package size: 0.5 mL
STORAGE / RECONSTITUTION
Reconstitute with 0.5 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower. After reconstitution, aliquot and store frozen; avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — detection of rat collagen type I by sandwich ELISA (dilution x1000). Use cat. 351 (rat collagen type I antigen) as the standard.
- IHC / histochemistry (frozen sections) — rat collagen-I histochemistry (dilution x20).
- Immunocytochemistry / ECM staining — immunostaining of collagen-I extracellular matrix in rat cell culture.
- Type-I-vs-III discrimination — cross-absorption against rat collagen III supports distinguishing type-I from type-III fibrils when paired with cat. 864.
- Western blot — used for rat collagen-I detection in published work (see References); native-oriented antibody with ~50% reactivity to the denatured chain.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Rat fibrosis models — collagen-I deposition in renal, hepatic, cardiac, pulmonary and dermal fibrosis (including CCl4 liver-injury models).
- Wound healing & scar maturation — type-I collagen turnover in rat repair models.
- Bone, tendon & cartilage ECM — type-I collagen mapping in musculoskeletal tissue.
- Collagen-scaffold tissue engineering — localisation / QC of collagen-I in rat scaffold and regenerative studies.
- Vascular & interstitial remodelling — type-I collagen in rat cardiovascular and interstitial ECM.
REFERENCES
Borkham-Kamphorst E, Kovalenko E, van Roeyen CR, Gassler N, Bomble M, Ostendorf T, Floege J, Gressner AM, Weiskirchen R. (2008) Platelet-derived growth factor isoform expression in carbon tetrachloride-induced chronic liver injury. Lab Invest 88: 1090-1100. (anti-rat collagen type I detection by Western blot)
