Mouse monoclonal antibody against human collagen type I, clone DBH 428, 1 mL. Highly type-I-specific — binds human collagen I with no reactivity to human collagen II, III, IV or V. The monoclonal capture partner for human collagen-I sandwich ELISA: pair with cat. 762 (goat anti-human collagen I polyclonal) on detection and use cat. 288 (human collagen I antigen) as the standard. Optimised for native-conformation work (ELISA, frozen-section IHC, cell culture) — the type-I-selective complement to the pan-fibrillar cat. 478 (mouse anti-collagen I/II/III, clone MCI-HA).
SPECIFICATIONS
Catalogue number: 393 | Host: Mouse | Target: Human
Target antigen: Human collagen type I
Gene family: COL1 (COL1A1 / COL1A2)
Clonality: Monoclonal | Clone: DBH 428 | Conjugate: Unconjugated
Specificity: Binds human collagen type I; no reactivity to human collagen II, III, IV or V. Native-conformation — not suitable for denaturing conditions.
Purification: Ammonium sulfate precipitation and anion-exchange chromatography
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M sodium chloride, 1% dextran, 1% mannitol, pH 8 | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody 10 days at +4 °C, or aliquot and freeze. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — sandwich-ELISA capture arm (dilution x1000). Pair with cat. 762 (goat polyclonal) on detection; use cat. 288 (human collagen I antigen) as the standard.
- IHC (frozen sections) — human collagen-I histochemistry (dilution x20).
- Cell culture / ECM staining — immunostaining of collagen-I in human cell culture.
- Type-I specificity — no cross-reactivity to collagen II / III / IV / V; the specific alternative to the pan-fibrillar cat. 478 (clone MCI-HA).
- Note: native-conformation antibody — not suitable for denatured Western blot or FFPE (HIER) workflows.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Human fibrosis & scar research — collagen-I quantification in dermal, hepatic, pulmonary, renal and cardiac fibrosis.
- Bone, tendon & cartilage biology — type-I collagen mapping in musculoskeletal tissue.
- Tumour stroma — collagen-I deposition by cancer-associated fibroblasts.
- Collagen-scaffold & tissue engineering — localisation / QC of collagen-I in scaffolds and regenerative constructs.
- Wound healing — type-I collagen turnover in repair models.
