Goat polyclonal antibody against native human collagen type I (COL1A1 / COL1A2), affinity-purified on immobilised human collagen I and cross-adsorbed against other human collagens, 1 mL. Type-I-selective — cross-reactivity to collagen III and IV below 5 %. The goat detection partner for human collagen-I sandwich ELISA — pair with the mouse mAb cat. 393 (clone DBH 428) on the capture arm and use cat. 288 (human collagen I antigen) as the standard — and for frozen-section IHC. Not suitable for denaturing conditions.
SPECIFICATIONS
Catalogue number: 762 | Host: Goat | Target: Human
Target antigen: Native human collagen type I (COL1A1 / COL1A2), triple-helical fold
Gene: COL1A1 (17q21.33), COL1A2 (7q21.3) | UniProt: P02452, P08123
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds human collagen I; cross-reactivity to human collagen III and IV below 5 %. Native-conformation — not suitable for denaturing / dissociating conditions.
Purification: Affinity-purified on immobilised human collagen I, cross-absorbed against other human collagens.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M NaCl, 1% dextran, 1% mannitol | Package size: 1 mL (reconstitute in 1 mL sterile water)
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — detection of human collagen I (starting dilution 1:3 000); pair with the mouse mAb cat. 393 on capture and use cat. 288 (human collagen I antigen) as the standard.
- IHC (frozen sections) — collagen-I histochemistry (starting dilution 1:20); no antigen retrieval typically required.
- Cell culture / ECM staining — collagen-I matrix deposited by fibroblasts and osteoblasts.
- Note: native-conformation antibody — not suitable for denaturing / dissociating conditions.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Fibrosis research — collagen-I deposition in dermal, hepatic, pulmonary, renal and cardiac fibrosis.
- Bone and tendon biology — type-I collagen in musculoskeletal tissue.
- Tumour stroma — collagen-I by cancer-associated fibroblasts.
- Collagen-scaffold / tissue-engineering QC.
