Mouse monoclonal antibody against human collagen types I, II and III, clone MCI-HA, IgM, 1 mL. Pan-fibrillar reagent binding native and heat-denatured human collagen I, II and III, with under 2% cross-reactivity to collagen IV/V and no reactivity to human plasma proteins. The broad-reactivity complement to the type-selective clones cat. 393 (collagen I, clone DBH 428) and cat. 380 (collagen III, clone MC3-HA); use the human collagen antigens cat. 288 (I), 210 (II) and 889 (III) as standards.
SPECIFICATIONS
Catalogue number: 478 | Host: Mouse | Target: Human
Target antigen: Human collagen types I, II and III (fibrillar collagens)
Gene family: COL1 / COL2 / COL3 (COL1A1, COL2A1, COL3A1)
Clonality: Monoclonal | Clone: MCI-HA | Isotype: IgM | Conjugate: Unconjugated
Specificity: Binds human collagen types I, II and III (native and heat-denatured); cross-reactivity to collagen IV and V below 2%; no reactivity to human plasma proteins.
Purification: PEG 6000 precipitation and anion-exchange chromatography
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M sodium chloride, 1% dextran, 1% mannitol, pH 8 | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C for up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA (dilution x1000) — pan-fibrillar collagen detection; use antigens cat. 288, 210 and 889 as standards.
- IHC, frozen sections (dilution x20) — acetone- or ethanol-fixed cryostat sections.
- Note — paraffin-embedded tissue gives less satisfactory staining, even after proteolytic or microwave retrieval.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Fibrosis research — total fibrillar-collagen deposition in dermal, hepatic, pulmonary and renal fibrosis.
- Connective-tissue biology — combined collagen I/II/III mapping in skin, cartilage and vessel wall.
- Wound healing — fibrillar-collagen turnover in repair models.
- Tumour stroma — collagen deposition by cancer-associated fibroblasts.
- Tissue engineering — QC and localisation of fibrillar collagens in scaffolds and constructs.
