Monoclonal mouse (murine)
antibodies (Clone MCI-HA, IgM fraction) to human Collagen type I, II
and III
Specificity
Binds Human Collagen type
I, II and III (native and heat-denatured). Cross-reactivity with
human collagen type IV and V below 2%. No reactivity to human plasma
proteins.
Preparation
PEG-6000 precipitation and
purification by anion-exchange chromatography
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1%mannitol, pH 8
Applications and
recommended dilutions
Acetone or ethanol-fixed cryostat
sections, results of staining of paraffin embedded tissues even after
treatment with proteolytic enzymes or microwave are less
satisfactory. ELISA (x1000), Immunohistology (x20)
Reconstitution
Use 1 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or
lower. Storage of reconstituted antibodies 10 days at +4°C.
