Rabbit polyclonal antibody against native human collagen types I, II, III, IV and V, affinity-purified on the immobilised mixed-collagen antigen and cross-absorbed against plasma proteins, 0.5 mL. A pan-collagen reagent that binds all five major human collagens (fibrillar I / II / III / V and network IV) in native conformation — for total-collagen ELISA and frozen-section IHC where broad collagen detection rather than isotype specificity is required. For isotype-specific detection, see cat. 287 (I), cat. 214 (II), cat. 153 (III), cat. 232 (IV) and cat. 201 (V). Not suitable for denaturing conditions.
SPECIFICATIONS
Catalogue number: 358 | Host: Rabbit | Target: Human
Target antigen: Native human collagen types I, II, III, IV and V (mixed-collagen immunogen)
Genes: COL1A1, COL2A1, COL3A1, COL4A1, COL5A1
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds human collagen I, II, III, IV and V; cross-absorbed against plasma proteins. Native-conformation — not suitable for denaturing / dissociating conditions.
Purification: Affinity-purified on immobilised human collagen I / II / III / IV / V, cross-absorbed against plasma proteins.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M NaCl, 1% dextran, 1% mannitol | Package size: 0.5 mL (reconstitute in 0.5 mL sterile water)
STORAGE / RECONSTITUTION
Reconstitute with 0.5 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — total-collagen detection (1:1 000).
- IHC (frozen sections) — broad collagen (I–V) histochemistry (1:10 to 1:20); no antigen retrieval typically required.
- Cell culture / ECM staining — total collagen-matrix deposition by fibroblasts and other ECM-producing cells.
- Note: native-conformation antibody — not suitable for denaturing conditions.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Total-collagen / general ECM detection — a single reagent covering the major collagen types.
- Fibrosis and scar research — broad collagen deposition.
- Combined basement-membrane and interstitial-collagen detection.
- Tissue-engineering and decellularised-scaffold total-collagen QC.
