Polyclonal rabbit antibody
anti-human collagen type I and III (COL1A1, COL3A1)
Specificity
Binds human collagen type I and III. Cross-reactivity to other human collagens as
measured by ELISA type IV below 3%, type V below 2%.
Preparation
Affinity purification on
immobilized human collagen type I and III with cross-absorption to
immobilised human blood plasma proteins and human collagen type II, IV and V.
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1%mannitol, pH 8
Applications and
recommended dilutions
Detection of human
collagen in ELISA (x4000), Immunohistochemistry (frozen sections) (x30), Immunostaining of
ECM in human cell cultures. Not suitable for denaturing and dissociating
conditions.
Reconstitution
Use 0.5 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or
lower
