Rabbit affinity-purified polyclonal antibody that recognises both rat collagen type I and type III, 1 mL. A single pan-fibrillar reagent for total interstitial (type I + III) collagen — the one-antibody readout that complements the type-specific pair cat. 376 (rabbit anti-rat collagen I) and cat. 864 (rabbit anti-rat collagen III). Optimised for native-conformation work: rat total-collagen sandwich ELISA, frozen-section histochemistry, and immunostaining of fibrillar-collagen ECM in cell culture.
SPECIFICATIONS
Catalogue number: 352 | Host: Rabbit | Target: Rat
Target antigen: Rat collagen type I and type III
Gene family: COL1 (COL1A1 / COL1A2) + COL3A1
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Recognises both rat collagen type I and type III (pan-fibrillar; not cross-absorbed). Native-conformation — not suitable for denaturing or dissociating conditions.
Purification: Affinity-purified on immobilised rat collagen type I and type III
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M sodium chloride, 1% dextran, 1% mannitol, pH 8 | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower. After reconstitution, aliquot and store frozen; avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — detection of rat collagen type I + III by sandwich ELISA (dilution x3000).
- IHC / histochemistry (frozen sections) — total fibrillar-collagen histochemistry (dilution x20).
- Immunocytochemistry / ECM staining — immunostaining of type I + III collagen ECM in cell culture.
- Type-specific alternative — for type-I-only or type-III-only readouts use the cross-absorbed cat. 376 (collagen I) or cat. 864 (collagen III).
- Note: native-conformation antibody — not suitable for denatured Western blot, FFPE (HIER), or other dissociating conditions.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Rat fibrosis models — total interstitial-collagen (I + III) burden in renal, hepatic, cardiac, pulmonary and dermal fibrosis.
- Wound healing & scar maturation — combined type I + III deposition across the repair timecourse.
- Tendon, ligament & musculoskeletal ECM — fibrillar-collagen mapping in rat connective tissue.
- Vascular & visceral remodelling — interstitial collagen in rat cardiovascular and visceral tissue.
- Collagen-scaffold tissue engineering — localisation / QC of fibrillar collagen in rat scaffold and regenerative studies.
